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PXD005159-5

PXD005159 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHeLa standard shotgun DDA analysis using a two-meter C18 monolithic silica column
DescriptionHuman HeLa proteome analysis by a nanoLC-MS/MS system using a two-meter C18 monolithic silica column under shallow gradient conditions without pre-fractionation was performed in this project. A hybrid quadrupole-orbitrap mass spectrometer (Thermo Q-Exactive) was used to analyze a tryptic digest of HeLa cells through this study. Identification results were filtered at 1% false discovery rate (FDR) thresholds by searching against a randomized decoy database using Mascot at peptide and protein levels.
HostingRepositoryjPOST
AnnounceDate2016-10-17
AnnouncementXMLSubmission_2018-09-18_23:39:53.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD005159
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterSaki Nambu
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxymethyl-L-cysteine; L-methionine sulfoxide
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-10-16 11:00:49ID requested
12016-10-16 19:22:57announced
22016-11-17 18:12:39announcedUpdated description.
32017-03-30 23:33:44announcedUpdated Repository support.
42017-03-31 00:09:03announcedUpdated DigitalObjectIdentifier.
52018-09-18 23:39:54announcedUpdated CV, FTP location and Modification list.
62021-02-04 17:07:11announced2021-02-04: Updated FTP location.
72022-09-18 02:58:30announced2022-09-18: Updated FTP location.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Monolithic silica column, HeLa cell
Contact List
Yasushi Ishihama
lab head
Saki Nambu
contact affiliationKyoto university
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://jpost.pharm.kyoto-u.ac.jp/repository/JPST000200