PXD005159-1
PXD005159 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | HeLa standard shotgun DDA analysis using a two-meter C18 monolithic silica column |
Description | Human HeLa proteome analysis by a nanoLC-MS/MS system using a two-meter C18 monolithic silica column under shallow gradient conditions without pre-fractionation was performed in this project. A hybrid quadrupole-orbitrap mass spectrometer (Thermo Q-Exactive) was used to analyze a tryptic digest of HeLa cells through this study. Peptide and protein assignments were filtered to a false discovery rates (FDR) of < 1% by searching against a randomized decoy database using Mascot. |
HostingRepository | jPOST |
AnnounceDate | 2016-10-17 |
AnnouncementXML | Submission_2016-10-16_19:22:56.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Saki Nambu |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetic acid derivatized residue; (2S,3R)-3-hydroxyaspartic acid; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2016-10-16 11:00:49 | ID requested | |
⏵ 1 | 2016-10-16 19:22:57 | announced | |
2 | 2016-11-17 18:12:39 | announced | Updated description. |
3 | 2017-03-30 23:33:44 | announced | Updated Repository support. |
4 | 2017-03-31 00:09:03 | announced | Updated DigitalObjectIdentifier. |
5 | 2018-09-18 23:39:54 | announced | Updated CV, FTP location and Modification list. |
6 | 2021-02-04 17:07:11 | announced | 2021-02-04: Updated FTP location. |
7 | 2022-09-18 02:58:30 | announced | 2022-09-18: Updated FTP location. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Monolithic silica column, HeLa cell |
Contact List
Yasushi Ishihama | |
---|---|
lab head | |
Saki Nambu | |
contact affiliation | Kyoto university |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |