PXD005159-4
PXD005159 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | HeLa standard shotgun DDA analysis using a two-meter C18 monolithic silica column |
| Description | Human HeLa proteome analysis by a nanoLC-MS/MS system using a two-meter C18 monolithic silica column under shallow gradient conditions without pre-fractionation was performed in this project. A hybrid quadrupole-orbitrap mass spectrometer (Thermo Q-Exactive) was used to analyze a tryptic digest of HeLa cells through this study. Identification results were filtered at 1% false discovery rate (FDR) thresholds by searching against a randomized decoy database using Mascot at peptide and protein levels. |
| HostingRepository | jPOST |
| AnnounceDate | 2016-10-17 |
| AnnouncementXML | Submission_2017-03-31_00:09:02.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD005159 |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Saki Nambu |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | iodoacetic acid derivatized residue; (2S,3R)-3-hydroxyaspartic acid; iodoacetamide derivatized residue |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2016-10-16 11:00:49 | ID requested | |
| 1 | 2016-10-16 19:22:57 | announced | |
| 2 | 2016-11-17 18:12:39 | announced | Updated description. |
| 3 | 2017-03-30 23:33:44 | announced | Updated Repository support. |
| ⏵ 4 | 2017-03-31 00:09:03 | announced | Updated DigitalObjectIdentifier. |
| 5 | 2018-09-18 23:39:54 | announced | Updated CV, FTP location and Modification list. |
| 6 | 2021-02-04 17:07:11 | announced | 2021-02-04: Updated FTP location. |
| 7 | 2022-09-18 02:58:30 | announced | 2022-09-18: Updated FTP location. |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Monolithic silica column, HeLa cell |
Contact List
| Yasushi Ishihama | |
|---|---|
| lab head | |
| Saki Nambu | |
| contact affiliation | Kyoto university |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |




