PXD001010-2

PXD001010 is an original dataset announced via ProteomeXchange.

Title	SWATH Analysis of Yeast Proteome over Time in Response to Osmotic Stress
Description	SWATH analysis of Yeast Proteome over time in response to Osmotic Stress. We sampled cell cultures in biological triplicates at six time points following the application of osmotic stress and acquired single injection DIA datasets on a high-resolution 5600 tripleTOF instrument operated in SWATH mode. Proteins were quantified by the targeted extraction and signal integration from the SWATH-MS datasets of peak groups representing proteotypic peptides for specific yeast proteins.
HostingRepository	PRIDE
AnnounceDate	2015-01-23
AnnouncementXML	Submission_2015-01-23_00:31:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lucia Espona Pernas
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	methionine oxidation with neutral loss of 64 Da; S-carboxamidomethyl-L-cysteine
Instrument	TSQ Quantum; TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2014-05-27 05:26:12	ID requested
1	2015-01-22 03:23:46	announced
⏵ 2	2015-01-23 00:31:10	announced	Updated project metadata.
3	2017-06-20 05:42:44	announced	Updated project metadata.
4	2017-10-24 00:14:19	announced	Updated project metadata.

Selevsek N, Chang CY, Gillet LC, Navarro P, Bernhardt OM, Reiter L, Cheng LY, Vitek O, Aebersold R, Reproducible and consistent quantification of the Saccharomyces cerevisiae proteome by SWATH-mass spectrometry. Mol Cell Proteomics, 14(3):739-49(2015) [pubmed]

curator keyword: Technical

submitter keyword: yeast, SWATH-MS, 5600, osmotic stress

Ruedi Aebersold
contact affiliation	Institute of Molecular Systems Biology, ETH Zurich
contact email	aebersold@imsb.biol.ethz.ch
lab head
Lucia Espona Pernas
contact affiliation	Institute of Molecular Systems Biology
contact email	espona@imsb.biol.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/01/PXD001010

PRIDE project URI

• PRIDE
  1. PXD001010
     1. Label: PRIDE project
     2. Name: SWATH Analysis of Yeast Proteome over Time in Response to Osmotic Stress