PXD000498-3

PXD000498 is an original dataset announced via ProteomeXchange.

Title	The quantitative and condition-dependent Escherichia coli proteome
Description	Measuring precise concentrations of proteins can provide insights into biological processes. Here we use efficient protein extraction and sample fractionation, as well as state-of-the-art quantitative mass spectrometry techniques to generate a comprehensive, condition-dependent protein-abundance map for Escherichia coli. We measure cellular protein concentrations for 55% of predicted E. coli genes (>2,300 proteins) under 22 different experimental conditions and identify methylation and N-terminal protein acetylations previously not known to be prevalent in bacteria. We uncover system-wide proteome allocation, expression regulation and post-translational adaptations. These data provide a valuable resource for the systems biology and broader E. coli research communities.
HostingRepository	PRIDE
AnnounceDate	2015-12-03
AnnouncementXML	Submission_2015-12-08_08:13:34.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000498
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alexander Schmidt
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	Oxidation; Acetyl; Carbamidomethyl
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2013-10-07 03:35:45	ID requested
1	2015-10-12 04:34:39	announced
2	2015-12-03 00:30:38	announced	Updated project metadata.
⏵ 3	2015-12-08 08:13:35	announced	Updated project metadata.

Schmidt A, Kochanowski K, Vedelaar S, Ahrn, é E, Volkmer B, Callipo L, Knoops K, Bauer M, Aebersold R, Heinemann M, The quantitative and condition-dependent Escherichia coli proteome. Nat Biotechnol, 34(1):104-10(2016) [pubmed]

curator keyword: Biological, Technical

submitter keyword: Absolute quantiifcation, iBAQ, mass spectreomtry, E. coli

Alexander Schmidt
contact affiliation	Biozentrum, University of Basel, 4056 Basel, Switzerland
contact email	alex.schmidt@unibas.ch
lab head
Alexander Schmidt
contact affiliation	Proteomics Core Facility
contact email	alex.schmidt@unibas.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/10/PXD000498

PRIDE project URI

• PRIDE
  1. PXD000498
     1. Label: PRIDE project
     2. Name: The quantitative and condition-dependent Escherichia coli proteome