PXD000498-1

PXD000498 is an original dataset announced via ProteomeXchange.

Title	The quantitative and condition-dependent Escherichia coli proteome
Description	The generation of dynamic models of biological processes critically depends on the determination of precise cellular concentrations of biomolecules. Here we report the cellular concentration for most expressed E. coli proteins reflecting 55% of the predicted genes (>2000 proteins) assessed under 22 different experimental conditions. The data were generated by combining efficient sample fractionation and state of the art quantitative mass spectrometry and represent the most comprehensive, condition-dependent protein abundance map for any organism to date. These data allowed us, for the first time, to define system-wide protein relationships between cell growth and protein expression across different conditions, defining proteome dynamics at different levels including functional, localization and posttranslational adaptations. We envision that the generated comprehensive quantitative E. coli proteome data, together with the identified protein modifications will become a major resource for the systems biology community and a great source for new discoveries of the broader E. coli community.
HostingRepository	PRIDE
AnnounceDate	2015-10-12
AnnouncementXML	Submission_2015-10-12_04:34:38.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000498
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alexander Schmidt
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	Oxidation; Acetyl; Carbamidomethyl
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2013-10-07 03:35:45	ID requested
⏵ 1	2015-10-12 04:34:39	announced
2	2015-12-03 00:30:38	announced	Updated project metadata.
3	2015-12-08 08:13:35	announced	Updated project metadata.

Dataset with its publication pending

curator keyword: Biological, Technical

submitter keyword: Absolute quantiifcation, iBAQ, mass spectreomtry, E. coli

Alexander Schmidt
contact affiliation	Biozentrum, University of Basel, 4056 Basel, Switzerland
contact email	alex.schmidt@unibas.ch
lab head
Alexander Schmidt
contact affiliation	Proteomics Core Facility
contact email	alex.schmidt@unibas.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/10/PXD000498

PRIDE project URI

• PRIDE
  1. PXD000498
     1. Label: PRIDE project
     2. Name: The quantitative and condition-dependent Escherichia coli proteome