PXD000498-2

PXD000498 is an original dataset announced via ProteomeXchange.

Title	The quantitative and condition-dependent Escherichia coli proteome
Description	Measuring precise concentrations of proteins can provide insights into biological processes. Here we use efficient protein extraction and sample fractionation, as well as state-of-the-art quantitative mass spectrometry techniques to generate a comprehensive, condition-dependent protein-abundance map for Escherichia coli. We measure cellular protein concentrations for 55% of predicted E. coli genes (>2,300 proteins) under 22 different experimental conditions and identify methylation and N-terminal protein acetylations previously not known to be prevalent in bacteria. We uncover system-wide proteome allocation, expression regulation and post-translational adaptations. These data provide a valuable resource for the systems biology and broader E. coli research communities.
HostingRepository	PRIDE
AnnounceDate	2015-12-03
AnnouncementXML	Submission_2015-12-03_00:30:37.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000498
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alexander Schmidt
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	Oxidation; Acetyl; Carbamidomethyl
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2013-10-07 03:35:45	ID requested
1	2015-10-12 04:34:39	announced
⏵ 2	2015-12-03 00:30:38	announced	Updated project metadata.
3	2015-12-08 08:13:35	announced	Updated project metadata.

Dataset with its publication pending

curator keyword: Biological, Technical

submitter keyword: Absolute quantiifcation, iBAQ, mass spectreomtry, E. coli

Alexander Schmidt
contact affiliation	Biozentrum, University of Basel, 4056 Basel, Switzerland
contact email	alex.schmidt@unibas.ch
lab head
Alexander Schmidt
contact affiliation	Proteomics Core Facility
contact email	alex.schmidt@unibas.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000498
     1. Label: PRIDE project
     2. Name: The quantitative and condition-dependent Escherichia coli proteome