PXD083748-1

PXD083748 is an original dataset announced via ProteomeXchange.

Title	Proteomic and Machine Learning Analyses Reveal the Mechanism of Baicalin in Inhibiting Biofilm Formation of Escherichia coli
Description	This study investigated the proteomic profiles of Escherichia coli under different MBIC treatment levels using data-independent acquisition (DIA) quantitative proteomics. The dataset included five experimental groups (A-E), with four biological replicates per group, resulting in a total of 20 biological samples. Group A was treated with 2× MBIC, group B with 1× MBIC, group C with 1/2× MBIC, group D with 1/4× MBIC, and group E with 0× MBIC. Peptides were analyzed by nanoLC-MS/MS using a Vanquish neo nano-UPLC system coupled to an Astral mass spectrometer. The acquired DIA data were processed using Spectronaut for protein identification and quantitative analysis. The dataset contains the raw mass spectrometry data together with the associated protein and peptide identification and quantification results.
HostingRepository	PRIDE
AnnounceDate	2026-09-08
AnnouncementXML	Submission_2026-09-08_01:05:19.970.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lei Wei
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: NEWT:562;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-09-05 13:02:49	ID requested
⏵ 1	2026-09-08 01:05:20	announced

Dataset with its publication pending

submitter keyword: DIA, Spectronaut,Escherichia coli, Astral, MBIC, quantitative proteomics, LC-MS/MS

Lei Wang
contact affiliation	Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences
contact email	wanglei03@caas.cn
lab head
Lei Wei
contact affiliation	Lanzhou Institute of Animal Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences
contact email	weilei0316@outlook.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD083748

PRIDE project URI

• PRIDE
  1. PXD083748
     1. Label: PRIDE project
     2. Name: Proteomic and Machine Learning Analyses Reveal the Mechanism of Baicalin in Inhibiting Biofilm Formation of Escherichia coli