PXD082390-1

PXD082390 is an original dataset announced via ProteomeXchange.

Title	Site-Specific Lysine Acetylation Regulates Bacteroides vulgatus Colonization and Enables Precision Restoration by Medicinal and Edible Homologous Polysaccharides
Description	LC-MS/MS Analysis peptide were enriched using anti-acetyl-lysine affinity resins, desalted with C18 ZipTips (Millipore), vacuum-dried, and reconstituted in 0.1% formic acid. Liquid chromatography was performed on an EASY-nLC 1200 system (Thermo Fisher Scientific) coupled to an Orbitrap Exploris 480 mass spectrometer equipped with a FAIMS Pro interface (Thermo Fisher Scientific). Peptides were separated on a reversed-phase analytical column at a flow rate of 300 nL/min using a linear acetonitrile gradient. Mass spectrometric data were acquired in data-dependent acquisition (DDA) mode. Full MS scans were collected over an m/z range of 350–1500 at a resolution of 60,000, followed by MS/MS acquisition at a resolution of 15,000 using higher-energy collisional dissociation (HCD). Dynamic exclusion and automatic gain control (AGC) settings followed the manufacturer's recommended parameters. Raw data were analyzed using Proteome Discoverer (version 2.4, Thermo Fisher Scientific) with the Sequest HT search engine against the Bacteroides vulgatus ATCC 8482 protein database downloaded from UniProt. Carbamidomethylation of cysteine was specified as a fixed modification, whereas methionine oxidation, protein N-terminal acetylation, and lysine acetylation were set as variable modifications. Peptide-spectrum matches were filtered at a false discovery rate (FDR) < 1% at both the peptide and protein levels.
HostingRepository	iProX
AnnounceDate	2026-08-08
AnnouncementXML	Submission_2026-08-10_19:02:21.330.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lishan Zhang
SpeciesList	scientific name: Bacteroides vulgatus ATCC 8482; NCBI TaxID: 435590;
ModificationList	acetylated residue
Instrument	4800 Plus MALDI TOF/TOF

Revision	Datetime	Status	ChangeLog Entry
0	2026-08-10 19:02:01	ID requested
⏵ 1	2026-08-10 19:02:21	announced

Dataset with its publication pending

submitter keyword: Bacteroides vulgatus, intestinal colonization, lysine acetylation, Ulcerative colitis, medicinal and edible homologous polysaccharides

Lishan Zhang
contact affiliation	Fujian University of Traditional Chinese Medicine
contact email	1215847994@qq.com
lab head
Lishan Zhang
contact affiliation	Fujian University of Traditional Chinese Medicine
contact email	1215847994@qq.com
dataset submitter

iProX dataset URI