LC-MS/MS Analysis peptide were enriched using anti-acetyl-lysine affinity resins, desalted with C18 ZipTips (Millipore), vacuum-dried, and reconstituted in 0.1% formic acid. Liquid chromatography was performed on an EASY-nLC 1200 system (Thermo Fisher Scientific) coupled to an Orbitrap Exploris 480 mass spectrometer equipped with a FAIMS Pro interface (Thermo Fisher Scientific). Peptides were separated on a reversed-phase analytical column at a flow rate of 300 nL/min using a linear acetonitrile gradient. Mass spectrometric data were acquired in data-dependent acquisition (DDA) mode. Full MS scans were collected over an m/z range of 350–1500 at a resolution of 60,000, followed by MS/MS acquisition at a resolution of 15,000 using higher-energy collisional dissociation (HCD). Dynamic exclusion and automatic gain control (AGC) settings followed the manufacturer's recommended parameters. Raw data were analyzed using Proteome Discoverer (version 2.4, Thermo Fisher Scientific) with the Sequest HT search engine against the Bacteroides vulgatus ATCC 8482 protein database downloaded from UniProt. Carbamidomethylation of cysteine was specified as a fixed modification, whereas methionine oxidation, protein N-terminal acetylation, and lysine acetylation were set as variable modifications. Peptide-spectrum matches were filtered at a false discovery rate (FDR) < 1% at both the peptide and protein levels.