PXD081656-1

PXD081656 is an original dataset announced via ProteomeXchange.

Title	Dual-species proteomic analysis of Pseudomonas aeruginosa and human airway epithelial cells during bacterial adhesion and intracellular infection
Description	Pseudomonas aeruginosa can adhere to and be internalized by airway epithelial cells, but the host and bacterial responses during bacterial adhesion and intracellular infection remain incompletely understood. In this study, we characterized the proteomes of P. aeruginosa PAO1 and CuFi-5 human airway epithelial cells carrying the cystic fibrosis-associated CFTR ΔF508 mutation. Experimental groups included uninfected CuFi-5 cells, planktonic PAO1, and CuFi-5 cells with adhered or intracellular PAO1. The experimental design included low- and high-calcium conditions. Proteomic profiles were determined using mass spectrometry-based label-free quantitative proteomics.
HostingRepository	PRIDE
AnnounceDate	2026-08-07
AnnouncementXML	Submission_2026-08-06_22:09:42.213.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yingxin Zhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Pseudomonas aeruginosa; NCBI TaxID: NEWT:287;
ModificationList	2-pyrrolidone-5-carboxylic acid (Gln); acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-25 14:00:33	ID requested
⏵ 1	2026-08-06 22:09:42	announced

Dataset with its publication pending

submitter keyword: CuFi-5, airway epithelial cells,Pseudomonas aeruginosa, host–pathogen interaction

Erika Lutter
contact affiliation	Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, Oklahoma, United States
contact email	erika.lutter@okstate.edu
lab head
Yingxin Zhang
contact affiliation	Oklahoma State University
contact email	yingxin.zhang@okstate.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD081656
     1. Label: PRIDE project
     2. Name: Dual-species proteomic analysis of Pseudomonas aeruginosa and human airway epithelial cells during bacterial adhesion and intracellular infection