PXD081558-1

PXD081558 is an original dataset announced via ProteomeXchange.

Title	Identification of TSC22D3-associated proteins in NK-92MI cells via immunoprecipitation-mass spectrometry
Description	This project aims to comprehensively identify the protein interactome of TSC22D3 (also known as GILZ) in the human natural killer cell line NK‑92MI using an immunoprecipitation‑based shotgun proteomics approach. Whole‑cell lysates were subjected to co‑immunoprecipitation with a specific anti‑TSC22D3 antibody and a matched rabbit IgG isotype control to discriminate specific interactors from non‑specific background binders. The immunoprecipitated protein complexes were processed using either in‑gel digestion (for Coomassie‑stained gel bands) or filter‑aided sample preparation (FASP) with trypsin digestion, depending on the sample type. The resulting peptide mixtures were separated by nano‑flow reversed‑phase liquid chromatography (EASY‑nLC) and analyzed on a Q Exactive HF‑X mass spectrometer operating in data‑dependent acquisition (DDA) mode with HCD fragmentation. Raw MS data were searched against the Homo sapiens Swiss‑Prot reference proteome (uniprot_homo_20250723) using Mascot 2.6 and Proteome Discoverer 2.4, with a peptide‑spectrum match (PSM) false discovery rate (FDR) of ≤ 0.01, allowing up to two missed tryptic cleavages, carbamidomethylation of cysteine as fixed modification, and oxidation of methionine and N‑terminal acetylation as variable modifications. This dataset will provide a high‑confidence molecular inventory of TSC22D3‑associated proteins, facilitating the elucidation of its regulatory functions in NK cell cytotoxicity, glucocorticoid signaling, and immune homeostasis.
HostingRepository	iProX
AnnounceDate	2026-07-22
AnnouncementXML	Submission_2026-07-23_19:34:56.784.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lele Sun
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-23 19:22:59	ID requested
⏵ 1	2026-07-23 19:34:57	announced

Dataset with its publication pending

submitter keyword: TSC22D3, GILZ, NK-92-MI, Interactome, Immunoprecipitation, LC-MS/MS, Protein-protein interaction

Hong Liu
contact affiliation	Dermatology Hospital of Shandong First Medical University
contact email	hongyue2519@hotmail.com
lab head
Lele Sun
contact affiliation	Dermatology Hospital of Shandong First Medical University
contact email	438065194@qq.com
dataset submitter

iProX dataset URI