This project aims to comprehensively identify the protein interactome of TSC22D3 (also known as GILZ) in the human natural killer cell line NK‑92MI using an immunoprecipitation‑based shotgun proteomics approach. Whole‑cell lysates were subjected to co‑immunoprecipitation with a specific anti‑TSC22D3 antibody and a matched rabbit IgG isotype control to discriminate specific interactors from non‑specific background binders. The immunoprecipitated protein complexes were processed using either in‑gel digestion (for Coomassie‑stained gel bands) or filter‑aided sample preparation (FASP) with trypsin digestion, depending on the sample type. The resulting peptide mixtures were separated by nano‑flow reversed‑phase liquid chromatography (EASY‑nLC) and analyzed on a Q Exactive HF‑X mass spectrometer operating in data‑dependent acquisition (DDA) mode with HCD fragmentation. Raw MS data were searched against the Homo sapiens Swiss‑Prot reference proteome (uniprot_homo_20250723) using Mascot 2.6 and Proteome Discoverer 2.4, with a peptide‑spectrum match (PSM) false discovery rate (FDR) of ≤ 0.01, allowing up to two missed tryptic cleavages, carbamidomethylation of cysteine as fixed modification, and oxidation of methionine and N‑terminal acetylation as variable modifications. This dataset will provide a high‑confidence molecular inventory of TSC22D3‑associated proteins, facilitating the elucidation of its regulatory functions in NK cell cytotoxicity, glucocorticoid signaling, and immune homeostasis.