PXD080696 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Interactome analysis of the Toxoplasma gondii effectors EAF1 and GRA35 by affinity purification and data-independent acquisition mass spectrometry |
| Description | Toxoplasma gondii is an obligate intracellular parasite that resides within a parasitophorous vacuole and secretes effector proteins that remodel the host cell. This dataset supports a study of the parasite effector EAF1 (TGGT1_225160), investigated as a candidate factor that recruit the host endosomal sorting complex required for transport (ESCRT) machinery to the parasitophorous vacuole membrane (PVM). Affinity purification was performed on epitope-tagged EAF1, with untagged wild-type and GRA35-HA parasites as controls, followed by quantitative data-independent acquisition mass spectrometry to define co-purifying host and parasite proteins. Comparative analysis of the tagged and control purifications was used to identify candidate interaction partners, with particular attention to components of the ESCRT-I subcomplex. The dataset comprises the mass spectrometer raw files, the Spectronaut search output, the protein sequence databases used, and the associated quality control, normalisation, and differential abundance results. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-13 |
| AnnouncementXML | Submission_2026-08-13_13:13:17.108.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Einar Olafsson |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Toxoplasma gondii RH-88; NCBI TaxID: NEWT:1208370; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-07-07 18:38:03 | ID requested | |
| ⏵ 1 | 2026-08-13 13:13:17 | announced | |
Publication List
| 10.64898/2026.07.08.737057; |
| Olafsson EB, Arnold CS, Kellermeier JA, Rimple PA, Kaur H, Wang Y, Sexton JZ, Sv, รค, rd S, Carruthers VB, O'Meara MJ, modulator of ESCRT subversion. bioRxiv, ():(2026) [pubmed] |
Keyword List
| submitter keyword: EAF1, MaxLFQ, affinity purification mass spectrometry, immunoprecipitation, data-independent acquisition, GRA35,Toxoplasma gondii, effector protein, Spectronaut, parasitophorous vacuole membrane, host-pathogen interaction, label-free quantification, ESCRT-I |
Contact List
| Einar Birnir Olafsson |
|---|
| contact affiliation | University of Michigan, Department of Microbiology & Immunology |
| contact email | olafsson@med.umich.edu |
| lab head | |
| Einar Olafsson |
|---|
| contact affiliation | University of Michigan |
| contact email | olafsson@med.umich.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD080696 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD080696
- Label: PRIDE project
- Name: Interactome analysis of the Toxoplasma gondii effectors EAF1 and GRA35 by affinity purification and data-independent acquisition mass spectrometry
