PXD077844 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Quantitative Temporal Phosphoproteomic Profiling of KBU2046 Treated Human Prostate Cancer Cells |
| Description | This project details a quantitative phosphoproteomic analysis of PC3 cells treated with the compound KBU2046. The study evaluated cellular responses to vehicle control, 1 µM KBU2046, and 10 µM KBU2046 at three time points (15 minutes, 1 hour, and 4 hours) using three biological replicates per condition. To enable robust comparative quantification, samples were multiplexed using Tandem Mass Tag 10 plex reagents. Phosphorylated peptides were enriched utilizing titanium dioxide affinity chromatography prior to analysis on an Orbitrap Eclipse Tribrid mass spectrometer operating in data-dependent acquisition mode with synchronous precursor selection MS3 quantitation. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-09-03 |
| AnnouncementXML | Submission_2026-09-03_12:30:44.109.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Weining Chen |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | Phospho; TMT6plex; Oxidation |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-04-29 23:44:11 | ID requested | |
| ⏵ 1 | 2026-09-03 12:30:44 | announced | |
Publication List
Keyword List
Contact List
| Raymond Bergan |
|---|
| contact affiliation | Director Stony Brook Cancer Center |
| contact email | Raymond.Bergan@stonybrookmedicine.edu |
| lab head | |
| Weining Chen |
|---|
| contact affiliation | University of Nebraska Medical Center |
| contact email | wchen@unmc.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD077844 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD077844
- Label: PRIDE project
- Name: Quantitative Temporal Phosphoproteomic Profiling of KBU2046 Treated Human Prostate Cancer Cells
