This project details a quantitative phosphoproteomic analysis of PC3 cells treated with the compound KBU2046. The study evaluated cellular responses to vehicle control, 1 µM KBU2046, and 10 µM KBU2046 at three time points (15 minutes, 1 hour, and 4 hours) using three biological replicates per condition. To enable robust comparative quantification, samples were multiplexed using Tandem Mass Tag 10 plex reagents. Phosphorylated peptides were enriched utilizing titanium dioxide affinity chromatography prior to analysis on an Orbitrap Eclipse Tribrid mass spectrometer operating in data-dependent acquisition mode with synchronous precursor selection MS3 quantitation.