PXD076994-1

PXD076994 is an original dataset announced via ProteomeXchange.

Title	Regulation of mechanical intercellular junctions and components of the mitochondrial electron transport chain and the TCA cycle in the hypertrophic heart by pyridostigmine or trandolapril
Description	The conventional approach to studying hypertension and cardiac hypertrophy primarily focuses on electrochemical signaling mediated by gap junctions. However, mechanical adherens junctions also contribute to hypertrophy through activation of β-catenin, promoting transcription of growth-inducing genes. Pyridostigmine reduces heart rate, whereas trandolapril is clinically employed as an antihypertensive agent. In this study, we investigated the impact of both drugs on the cardiac phosphoproteome of hypertensive spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto (WKY) rats. Label-free LC–MS quantification was used to identify alterations in biological processes and cellular compartments. Both agents induced changes in the phosphorylation of proteins associated with adherens junctions, desmosomes, the mitochondrial electron transport chain, and metabolic pathways, each displaying distinct drug-specific patterns. Our data indicate that adaptive remodeling in hypertensive hearts is largely governed by phosphorylation-mediated regulation of the entire area composita, particularly adherens and desmosomal junctions, highlighting the crucial role of mechanical junctions in addition to electrochemical signaling. The observed ability of trandolapril to restore phosphorylation of these junctional proteins, which may contribute to the suppression of cardiac hypertrophy, underscores the importance of preserving cardiomyocyte mechanical stability in the management of hypertension-related pathologies.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-07_04:21:24.073.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jana Jasprova
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-10 09:00:01	ID requested
⏵ 1	2026-09-07 04:21:25	announced

Drastichova Z, Hejnova L, Boro, š A, Behuliak M, Novotny J, Regulation of mechanical intercellular junctions and components of the mitochondrial electron transport chain and the TCA cycle in the hypertrophic heart by pyridostigmine or trandolapril. Front Mol Biosci, 13():1858563(2026) [pubmed]

10.3389/fmolb.2026.1858563;

submitter keyword: hypertrophy, pyridostigmine, trandolapril, electron transport chain, TCA cycle,SHR rats, desmosome, adherens junction, sarcomere

Zdenka Drastichova
contact affiliation	Department of Physiology, Faculty of Science, Charles University, 128 00 Prague, Czechia
contact email	zdenka.drastichova@natur.cuni.cz
lab head
Jana Jasprova
contact affiliation	Charles University, Faculty of Science
contact email	vanikova@natur.cuni.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD076994
     1. Label: PRIDE project
     2. Name: Regulation of mechanical intercellular junctions and components of the mitochondrial electron transport chain and the TCA cycle in the hypertrophic heart by pyridostigmine or trandolapril