The conventional approach to studying hypertension and cardiac hypertrophy primarily focuses on electrochemical signaling mediated by gap junctions. However, mechanical adherens junctions also contribute to hypertrophy through activation of β-catenin, promoting transcription of growth-inducing genes. Pyridostigmine reduces heart rate, whereas trandolapril is clinically employed as an antihypertensive agent. In this study, we investigated the impact of both drugs on the cardiac phosphoproteome of hypertensive spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto (WKY) rats. Label-free LC–MS quantification was used to identify alterations in biological processes and cellular compartments. Both agents induced changes in the phosphorylation of proteins associated with adherens junctions, desmosomes, the mitochondrial electron transport chain, and metabolic pathways, each displaying distinct drug-specific patterns. Our data indicate that adaptive remodeling in hypertensive hearts is largely governed by phosphorylation-mediated regulation of the entire area composita, particularly adherens and desmosomal junctions, highlighting the crucial role of mechanical junctions in addition to electrochemical signaling. The observed ability of trandolapril to restore phosphorylation of these junctional proteins, which may contribute to the suppression of cardiac hypertrophy, underscores the importance of preserving cardiomyocyte mechanical stability in the management of hypertension-related pathologies.