PXD076522-1

PXD076522 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	HDX-MS Analysis of VPS13C-dATG2C-3xFLAG-CaM +/- Calcium
Description	We carried out HDX-MS experiments on two conditions, VPS13C(dATG2C)-3xFLAG + EGTA, and VPS13C(dATG2C)-3xFLAG + Ca2+ to define conformational changes accompanying Ca2+. We saw an overall 69.7% coverage of VPS13C(dATG2C)-3xFLAG with 424 peptides identified and 66.4% coverage of CaM with 16 peptides identified. Looking at VPS13C(dATG2C)-3xFLAG, there is an exposure from 135-142 upon the addition of Ca2+. In CaM, there is an exposure from 73-85, and protections are seen at 91-103 and 125-141 upon the addition of Ca2+.
HostingRepository	PRIDE
AnnounceDate	2026-06-02
AnnouncementXML	Submission_2026-06-02_11:07:01.822.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	John Burke
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	impact II

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-02 11:58:54	ID requested
⏵ 1	2026-06-02 11:07:02	announced

Publication List

10.1101/2025.11.10.687702;
Li D, Wang X, Hu B, Hao H, Hamill S, Li Y, Chen G, De Camilli P, Reinisch KM, Insights into the regulation of VPS13 family bridge-like lipid transfer proteins from the structure of VPS13C. bioRxiv, ():(2025) [pubmed]

10.1101/2025.11.10.687702;

Li D, Wang X, Hu B, Hao H, Hamill S, Li Y, Chen G, De Camilli P, Reinisch KM, Insights into the regulation of VPS13 family bridge-like lipid transfer proteins from the structure of VPS13C. bioRxiv, ():(2025) [pubmed]

Keyword List

submitter keyword: None

submitter keyword: None

Contact List

John Burke
contact affiliation	University of Victoria
contact email	jeburke@uvic.ca
lab head
John Burke
contact affiliation	University of Victoria
contact email	jeburke@uvic.ca
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD076522
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD076522

PRIDE project URI

Repository Record List

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