We carried out HDX-MS experiments on two conditions, VPS13C(dATG2C)-3xFLAG + EGTA, and VPS13C(dATG2C)-3xFLAG + Ca2+ to define conformational changes accompanying Ca2+. We saw an overall 69.7% coverage of VPS13C(dATG2C)-3xFLAG with 424 peptides identified and 66.4% coverage of CaM with 16 peptides identified. Looking at VPS13C(dATG2C)-3xFLAG, there is an exposure from 135-142 upon the addition of Ca2+. In CaM, there is an exposure from 73-85, and protections are seen at 91-103 and 125-141 upon the addition of Ca2+.