PXD073675 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | PUS7 cytoplasmic localization directs a pseudouridine-mediated cellular stress response (CU-OOH) |
| Description | Pseudouridine is an abundant post-transcriptional modification found across all classes of RNA. Since its discovery in mRNAs a decade ago, it has been widely speculated that Y might govern additional post-transcriptional regulation of gene expression. Here, we demonstrate that one of the principal enzymes responsible for adding Y to mRNAs, pseudouridine synthase 7 (PUS7), accumulates in the cytoplasm under a variety of stress conditions in Saccharomyces cerevisiae and BEAS-2B human epithelial lung cells. The localization of PUS7 to the cytoplasm promotes Y-incorporation into hundreds of different mRNA sequences and increases cellular fitness under ROS and divalent metal ion stress. The preponderance of newly identified Y-sites lies within portions of the mRNA important for post-transcriptional control—-coding regions and 3’ UTRs. Quantitative proteomics reveal that shifts in the cellular post-transcriptional modification landscape upon PUS7 relocalization reshapes the proteome. Our data suggest a mechanism whereby stressors localize PUS7 in the cytoplasm to enable the direct modification and regulation of stress response mRNAs, thereby protecting cells from further stress-induced damage. (PUS7-NES_CU_1A 75059 PUS7-NES_CU_1B 75060 PUS7-NES_CU_1C 75061 PUS7-NLS_CU_2A 75062 PUS7-NLS_CU_2B 75063 PUS7-NLS_CU_2C 75064 PUS7-WT_CU_3A 75065 PUS7-WT_CU_3B 75066 PUS7-WT_CU_3C 75067)) |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-07 |
| AnnouncementXML | Submission_2026-04-07_02:50:46.965.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Minli Ruan |
| SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-01-27 17:13:09 | ID requested | |
| 1 | 2026-01-28 01:21:09 | announced | |
| 2 | 2026-04-07 02:49:58 | announced | 2026-04-07: Updated project metadata. |
| ⏵ 3 | 2026-04-07 02:50:47 | announced | 2026-04-07: Updated project metadata. |
| 4 | 2026-04-09 00:46:03 | announced | 2026-04-09: Updated project metadata. |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: PUS7, localization, stress response,Pseudouridine |
Contact List
| Kristin Koutmou |
|---|
| contact affiliation | University of Michigan |
| contact email | kkoutmou@umich.edu |
| lab head | |
| Minli Ruan |
|---|
| contact affiliation | University of Michigan |
| contact email | ruanminli@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD073675
- Label: PRIDE project
- Name: PUS7 cytoplasmic localization directs a pseudouridine-mediated cellular stress response (CU-OOH)
