PXD073675-1

PXD073675 is an original dataset announced via ProteomeXchange.

Title	PUS7 cytoplasmic localization directs a pseudouridine-mediated cellular stress response (CU-OOH)
Description	Pseudouridine is an abundant post-transcriptional modification found across all classes of RNA. Since its discovery in mRNAs a decade ago, it has been widely speculated that Y might govern additional post-transcriptional regulation of gene expression. Here, we demonstrate that one of the principal enzymes responsible for adding Y to mRNAs, pseudouridine synthase 7 (PUS7), accumulates in the cytoplasm under a variety of stress conditions in Saccharomyces cerevisiae and BEAS-2B human epithelial lung cells. The localization of PUS7 to the cytoplasm promotes Y-incorporation into hundreds of different mRNA sequences and increases cellular fitness under ROS and divalent metal ion stress. The preponderance of newly identified Y-sites lies within portions of the mRNA important for post-transcriptional control—-coding regions and 3’ UTRs. Quantitative proteomics reveal that shifts in the cellular post-transcriptional modification landscape upon PUS7 relocalization reshapes the proteome. Our data suggest a mechanism whereby stressors localize PUS7 in the cytoplasm to enable the direct modification and regulation of stress response mRNAs, thereby protecting cells from further stress-induced damage.
HostingRepository	PRIDE
AnnounceDate	2026-01-28
AnnouncementXML	Submission_2026-01-28_01:21:08.868.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Minli Ruan
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-27 17:13:09	ID requested
⏵ 1	2026-01-28 01:21:09	announced

Dataset with its publication pending

submitter keyword: PUS7, localization, stress response,Pseudouridine

Minli Ruan
contact affiliation	Graduate Research Assistant @ University of Michigan | RNA Biology, Nanopore Sequencing
contact email	mlruan@umich.edu
lab head
Minli Ruan
contact affiliation	University of Michigan
contact email	ruanminli@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD073675
     1. Label: PRIDE project
     2. Name: PUS7 cytoplasmic localization directs a pseudouridine-mediated cellular stress response (CU-OOH)