PXD071635-1

PXD071635 is an original dataset announced via ProteomeXchange.

Title	Beyond Repression: ArsR Functions as a Global Activator of Metabolic and Redox Responses in Escherichia Coli
Description	The arsenic-responsive repressor, ArsR, has long been understood as a canonical regulator of the arsRBC operon, which confers resistance to arsenic stress. However, recent studies suggest a broader regulatory scope for ArsR. Here, we investigated the proteomic landscape of Escherichia coli strains with and without ArsR to elucidate ArsR as an activator in both non-stressing and arsenic-stressing conditions. Using mass spectrometry-based shotgun proteomics and statistical analyses, we characterized the differential abundance of proteins across AW3110 (ΔarsRBC), AW3110 complemented with arsR, and wild-type K-12 strains under control and arsenite-stressed conditions. Our study shows ArsR influences proteomic networks beyond the ars operon, integrating metabolic and redox responses crucial for cellular adaptation and survival. This suggests that ArsR has a significant role in gut microbiome metabolomic profiles in response to arsenite. Proteins involved in alanine, lactaldehyde, arginine, thioredoxin, and proline pathways were significantly elevated in strains where ArsR was detected, both with and without arsenite. We identified proteins exhibiting an “ArsR-dependent” activation pattern, highlighting ArsR’s potential role in redox balance and energy metabolism. These findings challenge the classical view of ArsR as a re-pressor and position it as a pleiotropic regulator including broad activation.
HostingRepository	PRIDE
AnnounceDate	2026-02-02
AnnouncementXML	Submission_2026-02-01_16:36:37.268.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD071635
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Monika Tokmina-Lukaszewska
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: NEWT:562;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-05 05:32:18	ID requested
⏵ 1	2026-02-01 16:36:38	announced

10.3390/proteomes14010001;

10.6019/PXD071635;

Sather B, Larson J, Hutt Vater K, Westrum J, McDermott TR, Bothner B, . Proteomes, 14(1):(2026) [pubmed]

submitter keyword: mass spectrometry based proteomic analysis, arsenic stress response, Ars operon expression,arsR regulation, Escherichia coli proteome, proteome stress response, differential proteome analysis, proteomic pathway analysis

Brian Bothner
contact affiliation	Department of Chemisry & Biochemistry at Montanan State University, Bozeman, MT
contact email	bbothner@montana.edu
lab head
Monika Tokmina-Lukaszewska
contact affiliation	Montana State University
contact email	tokminalukas@montana.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD071635
     1. Label: PRIDE project
     2. Name: Beyond Repression: ArsR Functions as a Global Activator of Metabolic and Redox Responses in Escherichia Coli