PXD071583-1

PXD071583 is an original dataset announced via ProteomeXchange.

Title	Hexosamine Biosynthetic Pathway-Induced Cytoskeletal Remodeling: A Potential Driver for Early-Stage Non-Small Cell Lung Cancer
Description	Non-Small Cell Lung Cancer (NSCLC) is the primary cause of cancer-related deaths in the Philippinesworldwide. To alleviate this disease burden, development of biomarker screening for NSCLC has been proposed to facilitate earlier detection, disease management, and pathway treatment monitoring is urgently needed. However, Asians have been identified to have a distinct molecular signature for lung cancer as opposed to western demographic groups. A Biomarker discovery through quantitative phosphoproteomics approach focused on a Filipino cohort was thereby done on paired tumortumors and normal lung samples to allow for the identification of dysregulated proteins and their associated pathways. These samples were verified to have distinct protein expressions through Principal Component Analysis (PCA). A total of 2607 phosphoproteins were identified with 481 of these being significantly phosphorylatedfound to have aberrant phosphorylation in the tumor specimens. This study identified The 296 proteins upregulated with significantly increased phosphorylation and 185 proteins with downregulated significantly increased dephosphorylation. proteins were then subjected to fFunctional analysis . Enrichment showed that the differentially phosphorylated proteins were primarily involved with the cytoskeleton and metabolism pathways. By analyzing the phosphosite level modifications, we propose that thatidentified the possible role of proteins involved in the hexosamine biosynthetic pathway potentially in driving e downstream cytoskeletal remodeling via the activation of Rho GTPases. These proteins may then serve as possible biomarkers for NSCLC and targets for drug development.
HostingRepository	PRIDE
AnnounceDate	2026-09-09
AnnouncementXML	Submission_2026-09-09_10:41:31.781.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD071583
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Baby Rorielyn Dimayacyac-Esleta
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-04 10:41:21	ID requested
⏵ 1	2026-09-09 10:41:32	announced

10.6019/PXD071583;

Dimayacyac-Esleta BRT, Su, ñ, ga LBL, Zarate LM, Mira FD, Porras BJO, Juntilla DLA, Pondevida VB, Prieto EI, Phosphoproteomic Profiling of Early-Stage Non-Small Cell Lung Cancer Provides Preliminary Evidence of Phosphorylation-Regulated Rho GTPase Signaling Driving Cytoskeletal Remodeling, Angiogenesis, and Cell Cycle Progression. J Proteome Res, 25(9):4957-4972(2026) [pubmed]

10.1021/acs.jproteome.6c00453;

submitter keyword: NSCLC, phosphoproteomics,human

Baby Rorielyn Dimayacyac-Esleta
contact affiliation	Clinical Proteomics Laboratory, Institute of Chemistry, UPD
contact email	btdimayacyacesleta@up.edu.ph
lab head
Baby Rorielyn Dimayacyac-Esleta
contact affiliation	Institute of Chemistry, University of the Philippines-Diliman
contact email	cpl.upd@up.edu.ph
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD071583

PRIDE project URI

• PRIDE
  1. PXD071583
     1. Label: PRIDE project
     2. Name: Hexosamine Biosynthetic Pathway-Induced Cytoskeletal Remodeling: A Potential Driver for Early-Stage Non-Small Cell Lung Cancer