Non-Small Cell Lung Cancer (NSCLC) is the primary cause of cancer-related deaths in the Philippinesworldwide. To alleviate this disease burden, development of biomarker screening for NSCLC has been proposed to facilitate earlier detection, disease management, and pathway treatment monitoring is urgently needed. However, Asians have been identified to have a distinct molecular signature for lung cancer as opposed to western demographic groups. A Biomarker discovery through quantitative phosphoproteomics approach focused on a Filipino cohort was thereby done on paired tumortumors and normal lung samples to allow for the identification of dysregulated proteins and their associated pathways. These samples were verified to have distinct protein expressions through Principal Component Analysis (PCA). A total of 2607 phosphoproteins were identified with 481 of these being significantly phosphorylatedfound to have aberrant phosphorylation in the tumor specimens. This study identified The 296 proteins upregulated with significantly increased phosphorylation and 185 proteins with downregulated significantly increased dephosphorylation. proteins were then subjected to fFunctional analysis . Enrichment showed that the differentially phosphorylated proteins were primarily involved with the cytoskeleton and metabolism pathways. By analyzing the phosphosite level modifications, we propose that thatidentified the possible role of proteins involved in the hexosamine biosynthetic pathway potentially in driving e downstream cytoskeletal remodeling via the activation of Rho GTPases. These proteins may then serve as possible biomarkers for NSCLC and targets for drug development.