PXD069230-1

PXD069230 is an original dataset announced via ProteomeXchange.

Title	Discovery of Novel TRIM7-Associated Proteins Using Co-Immunoprecipitation Combined with Quantitative Mass Spectrometry
Description	This project aims to identify TRIM7's potential binding proteins using Co-IP and MS. Cell lysates with endogenous/exogenous TRIM7 will be incubated with TRIM7-specific antibody-conjugated agarose beads to immunoprecipitate TRIM7 complexes. Non-specific proteins are removed by stringent washing. Co-immunoprecipitated proteins are eluted, separated by SDS-PAGE, digested with trypsin, and analyzed by LC-MS/MS for identification. Bioinformatics analyses, such as GO annotation, KEGG pathway enrichment, and PPI network construction, will predict the functions of these binding proteins and their roles in TRIM7-related biological processes.
HostingRepository	iProX
AnnounceDate	2025-10-09
AnnouncementXML	Submission_2025-11-23_17:32:58.877.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fengjuan Yan
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-09 01:05:44	ID requested
⏵ 1	2025-11-23 17:32:59	announced

Dataset with its publication pending

submitter keyword: TRIM7, Co-IP, LC-MS/MS, KEGG Pathway Enrichment

Fengjuan Yan
contact affiliation	Jiangsu Normal University
contact email	yanfengjuan@whu.edu.cn
lab head
Fengjuan Yan
contact affiliation	Jiangsu Normal University
contact email	yanfengjuan@whu.edu.cn
dataset submitter

iProX dataset URI