This project aims to identify TRIM7's potential binding proteins using Co-IP and MS. Cell lysates with endogenous/exogenous TRIM7 will be incubated with TRIM7-specific antibody-conjugated agarose beads to immunoprecipitate TRIM7 complexes. Non-specific proteins are removed by stringent washing. Co-immunoprecipitated proteins are eluted, separated by SDS-PAGE, digested with trypsin, and analyzed by LC-MS/MS for identification. Bioinformatics analyses, such as GO annotation, KEGG pathway enrichment, and PPI network construction, will predict the functions of these binding proteins and their roles in TRIM7-related biological processes.