PXD068251 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Human glioblastoma derived cell line (U251) LC-MS |
| Description | This study investigated the molecular mechanisms of specific medium chain fatty acids in a cellular model of cancer. These fatty acids are provided in the medium chain triglyceride (MCT) ketogenic diet, that is a widely used treatment for patients with drug resistant epilepsy, where the diet is commonly considered to function through the production of ketones. Ketogenic diets are also increasingly being investigated for the treatment of various cancers. One recent advance in the field was the identification of several therapeutic mechanisms for one of these fatty acids, decanoic acid, independent of ketosis. In addition, a recent clinical trial of a new MCT blend containing elevated levels of decanoic acid provided seizure control without ketosis, confirming a ketone-independent therapeutic mechanism. The cellular role of this new MCT blend in cancer treatment has not been examined. Here, we investigate decanoic acid and the new MCT blend on cancer cell biology using U251 cells, a human glioblastoma-derived cancer line, treated in culture with therapeutically relevant concentrations of decanoic acid and the new MCT blend, for a period of time that has been shown to trigger metabolic changes in patients. The cellular mechanism of these medium chain fatty acids where then investigated a proteomic level. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-05-25 |
| AnnouncementXML | Submission_2026-05-24_18:50:48.339.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Steven Lynham |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-09-10 04:28:18 | ID requested | |
| ⏵ 1 | 2026-05-24 18:50:49 | announced | |
Publication List
| 10.1186/s12885-026-15909-3; |
| Pain E, Singh PK, Khan Y, Mears G, Yang X, Lynham S, Devlin PF, Rutherford T, Jones KL, Walker MC, Beesley P, Gentry MS, Williams RS, Metabolic targeting of pantothenate by medium chain fats in glioblastoma cells. BMC Cancer, 26(1):(2026) [pubmed] |
Keyword List
| submitter keyword: glioblastoma, decanoic acid, GBM, mechanistic study, fatty acid metabolism, medium chain triglycerides (MCT),Cancer |
Contact List
| Robin SB Williams |
|---|
| contact affiliation | Bourne Laboratory, Department of Biological Science, Royal Holloway University of London, Egham, Surrey, TW20 OEX, UK |
| contact email | robin.williams@rhul.ac.uk |
| lab head | |
| Steven Lynham |
|---|
| contact affiliation | King's College London |
| contact email | steven.lynham@kcl.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD068251 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD068251
- Label: PRIDE project
- Name: Human glioblastoma derived cell line (U251) LC-MS
