PXD067229-1

PXD067229 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of whole-cell, soma and neuronal projection fractions from day 35 iNeurons in Control and ASAH1-/-
Description	This experiment describes the quantitative proteomic profiling of whole-cell, soma, and neuronal projection fractions from day 35 induced neurons (iN) derived from control and ASAH1 knockout human embryonic stem cells. Soma and projection compartments were physically separated using Transwell culture inserts, and triplicate biological replicates were processed using TMTpro 18-plex labeling and high-resolution Orbitrap mass spectrometry with FAIMS. Data were searched against the human proteome with stringent FDR control and reporter ion quantification, enabling compartment-resolved analysis of proteome alterations associated with ASAH1 deficiency.
HostingRepository	PRIDE
AnnounceDate	2025-08-12
AnnouncementXML	Submission_2025-08-12_04:39:29.787.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Felix Kraus
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-11 15:02:48	ID requested
⏵ 1	2025-08-12 04:39:30	announced

Dataset with its publication pending

submitter keyword: PD, iN, iDA, lysosome dysfunction, neurodegeneration,TMTpro, projection proteomics

J. Wade Harper
contact affiliation	Department of Cell Biology, Harvard Medical School, Boston MA, USA
contact email	wade_harper@hms.harvard.edu
lab head
Felix Kraus
contact affiliation	Harvard Medical Sschool
contact email	felix_kraus@hms.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD067229

PRIDE project URI

• PRIDE
  1. PXD067229
     1. Label: PRIDE project
     2. Name: Proteomics analysis of whole-cell, soma and neuronal projection fractions from day 35 iNeurons in Control and ASAH1-/-