PXD066843 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Site-specific N-glycoproteomic analysis of extracellular vesicles from seminal plasma for the molecular typing of azoospermia |
| Description | This dataset contains mass spectrometry raw files and identification results from intact N-glycoproteomic analysis of extracellular vesicles (EVs) isolated from human seminal plasma. Intact N-glycopeptides were enriched and analyzed using an Orbitrap Fusion Lumos mass spectrometer coupled to an Easy-nLC 1200 system. The uploaded dataset includes: (1) Data-dependent acquisition (DDA) LC-MS/MS files acquired via stepped collision energy higher-energy collision dissociation (StepHCD) across discovery cohort samples; and (2) StepHCD-based parallel reaction monitoring (PRM) targeted LC-MS/MS files across validation cohort samples. Raw data files were searched against the human UniProt reviewed database using Glyco-Decipher software with a 1% false discovery rate (FDR). |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-24 |
| AnnouncementXML | Submission_2026-08-24_02:18:28.351.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Mingyang Li |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | deamidated residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-07-31 22:49:04 | ID requested | |
| 1 | 2026-08-14 20:16:09 | announced | |
| ⏵ 2 | 2026-08-24 02:18:28 | announced | 2026-08-24: Updated project metadata. |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: intact glycoproteomic,Azoospermia, seminal plasma, extracellular vesicles |
Contact List
| Xuejiang Guo |
|---|
| contact affiliation | State Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, Nanjing Medical University, Nanjing, 211166, China |
| contact email | guo_xuejiang@njmu.edu.cn |
| lab head | |
| Mingyang Li |
|---|
| contact affiliation | Southeast University |
| contact email | 230229239@seu.edu.cn |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD066843 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD066843
- Label: PRIDE project
- Name: Site-specific N-glycoproteomic analysis of extracellular vesicles from seminal plasma for the molecular typing of azoospermia
