PXD066843-1

PXD066843 is an original dataset announced via ProteomeXchange.

Title	Site-specific N-glycoproteomic analysis of extracellular vesicles from seminal plasma for the molecular typing of azoospermia
Description	This project presents an in-depth site-specific N-glycoproteomic analysis of extracellular vesicles (EVs) isolated from human seminal plasma, aiming to identify diagnostic biomarkers for the molecular typing of azoospermia. Using an optimized EVGlyco platform, we characterized 8,435 site-specific N-glycans from 394 N-glycoproteins in EVs, with targeted validation via parallel reaction monitoring (PRM) confirming key glycan signatures (e.g., MPO-N355-H6N2, ACP3-N94-H5N4F1, and CLU-N374-H10N9F1) that distinguish obstructive azoospermia (OA) from nonobstructive azoospermia (NOA) and its subtypes (hypospermatogenesis, maturation arrest, Sertoli cell-only syndrome). The dataset includes mass spectrometry raw files of intact N-glycopeptides from 110 seminal plasma samples (discovery and validation cohorts), supporting the potential of EV site-specific N-glycans as non-invasive biomarkers for azoospermia typing.
HostingRepository	PRIDE
AnnounceDate	2026-08-15
AnnouncementXML	Submission_2026-08-14_20:16:08.776.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mingyang Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-31 22:49:04	ID requested
⏵ 1	2026-08-14 20:16:09	announced
2	2026-08-24 02:18:28	announced	2026-08-24: Updated project metadata.

Dataset with its publication pending

submitter keyword: intact glycoproteomic,Azoospermia, seminal plasma, extracellular vesicles

Xuejiang Guo
contact affiliation	State Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, Nanjing Medical University, Nanjing, 211166, China
contact email	guo_xuejiang@njmu.edu.cn
lab head
Mingyang Li
contact affiliation	Southeast University
contact email	230229239@seu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD066843

PRIDE project URI

• PRIDE
  1. PXD066843
     1. Label: PRIDE project
     2. Name: Site-specific N-glycoproteomic analysis of extracellular vesicles from seminal plasma for the molecular typing of azoospermia