PXD066369-1

PXD066369 is an original dataset announced via ProteomeXchange.

Title	LC-MSMS data obtained from proteome extracted from whole cell lysate and lipid droplet isolated from mice brain tissue of Sham and LCMV injected mice at 2 and 4 days post infection
Description	To further characterize the potential role of LDs in viral infection we developed techniques to cleanly extract LDs from whole murine brains and performed comparative mass spectrometry profiling of proteins differentially associated with LCMV-LDs or SHAM-LDs isolated from the brain tissues of mice. Mice were infected with LCMV intracranially, and their brains were harvested at 2- and 4-days post infection (dpi), modelling early-stage infection, as evidenced by increases in viral load and cytokine expression.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-06_16:47:12.895.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zahra Telikani
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-21 18:18:14	ID requested
⏵ 1	2026-09-06 16:47:13	announced

Monson EA, Telikani Z, Laws JL, Milligan AJ, Amarasinghe I, Bamford SE, Rozario AM, Smith ML, Tran V, Dinh QN, Williamson N, Mechler A, Liu B, Johnson CJ, Pigram PJ, Leeming M, Nie S, Holien JK, Hofer MJ, Whelan DR, Helbig KJ, Integrative proteomics and lipidomics reveals dual roles for lipid droplets in the host cell antiviral response. Nat Commun, 17(1):(2026) [pubmed]

10.1038/s41467-026-74016-w;

submitter keyword: LC-MSMS, Brain, Lipid droplet,Mice, Whole cell lysate

Karla Jane Helbig
contact affiliation	Department of Microbiology, Anatomy, Physiology and Pharmacology, La Trobe University, Bundoora, Victoria, Australia La Trobe Institute for Molecular Science, La Trobe University, Bundoora, Victoria, Australia
contact email	K.Helbig@latrobe.edu.au
lab head
Zahra Telikani
contact affiliation	1 Department of Microbiology, Anatomy, Physiology and Pharmacology, La Trobe University, Bundoora, Victoria, Australia 2 La Trobe Institute for Molecular Science, La Trobe University, Bundoora, Victoria, Australia
contact email	za.telikani@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD066369

PRIDE project URI

• PRIDE
  1. PXD066369
     1. Label: PRIDE project
     2. Name: LC-MSMS data obtained from proteome extracted from whole cell lysate and lipid droplet isolated from mice brain tissue of Sham and LCMV injected mice at 2 and 4 days post infection