To further characterize the potential role of LDs in viral infection we developed techniques to cleanly extract LDs from whole murine brains and performed comparative mass spectrometry profiling of proteins differentially associated with LCMV-LDs or SHAM-LDs isolated from the brain tissues of mice. Mice were infected with LCMV intracranially, and their brains were harvested at 2- and 4-days post infection (dpi), modelling early-stage infection, as evidenced by increases in viral load and cytokine expression.