PXD066360-1

PXD066360 is an original dataset announced via ProteomeXchange.

Title	Structural and Functional Analysis of ASFV pI73R Reveals GNB1 Binding and Host Gene Modulation
Description	African swine fever virus (ASFV) causes a highly fatal disease in domestic pigs, resulting in substantial economic losses to the global swine industry. Vaccine development continues to be hindered by limited characterization of viral proteins and their functional redundancies. In this study, we employ combined experimental and computational approaches to characterize the ASFV pI73R protein, which contains a Z-DNA binding domain and plays a critical role in ASFV virulence and pathogenesis. We demonstrate that pI73R shares significant structural similarity with transcription factors of the forkhead box (FOX) protein family. Overexpression of pI73R results in downregulation of CRNKL1, a core spliceosome component, suggesting a potential mechanism by which pI73R modulates host protein synthesis. Using high-resolution mass spectrometry, we map the pI73R interactome and identify the host protein GNB1 as a novel direct interactor of pI73R which may facilitate its nuclear transport. Furthermore, we show that pI73R exhibits consistent oligomerization and expression across different ASFV genotypes, highlighting its functional importance. Taken together, these results provide new insights into pI73R function, ASFV-host dynamics, and offer promising directions for antiviral strategy development.
HostingRepository	PRIDE
AnnounceDate	2026-01-05
AnnouncementXML	Submission_2026-01-04_17:18:19.179.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD066360
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Katarzyna Dolata
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: NEWT:9825;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-21 04:27:05	ID requested
⏵ 1	2026-01-04 17:18:20	announced

Dolata KM, Bettin B, K, ü, chler R, Pannhorst K, Ushakov DS, Fuchs W, Karger A, Structural and Functional Analysis of ASFV pI73R Reveals GNB1 Binding and Host Gene Modulation. Int J Mol Sci, 26(24):(2025) [pubmed]

10.6019/PXD066360;

10.3390/ijms262411768;

submitter keyword: GNB1, ASFV, virus-host interaction,African swine fever virus, protein-protein interaction, pI73R, gene expression

Katarzyna Dolata
contact affiliation	Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, 17493 Greifswald-Insel Riems,Germany
contact email	katarzyna.dolata@fli.de
lab head
Katarzyna Dolata
contact affiliation	Friedrich-Loeffler Institut
contact email	katarzyna.dolata@fli.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD066360
     1. Label: PRIDE project
     2. Name: Structural and Functional Analysis of ASFV pI73R Reveals GNB1 Binding and Host Gene Modulation