PXD065940-1

PXD065940 is an original dataset announced via ProteomeXchange.

Title	In depth proteome analyais of primary human nasal epithelium induced by diesel exhaust particles
Description	Diesel exhaust particles (DEPs), a major component of airborne particulate matter (PM), are strongly associated with the development of both acute and chronic upper airway inflammatory diseases. In this study, we investigated the effects of prolonged daily exposure to low-dose DEPs on the maturation of primary human nasal epithelium (PHNE). PHNE cultured under air-liquid interface (ALI) conditions were exposed to 0.5μg cm-² of DEPs for 5 hours daily from ALI day 1 to day 23. PHNE samples were subsequently collected on ALI days 6, 12, and 24 and analyzed using mass spectrometry-based label-free quantitative proteomics. A total of 7,993 proteins were identified, with approximately half exhibiting time-dependent differential expression patterns throughout the course of PHNE maturation. To determine the proteins differentially expressed in response to DEP exposure, we conducted paired Student’s t-tests at each time point. Functional annotation clustering of these differentially expressed proteins revealed enrichment of gene ontology (GO) terms related to the inflammatory response on ALI day 6. By ALI day 24, several GO biological process (GO-BP) terms associated with cilia—such as cilium assembly, intraciliary transport, and cilium movement—were significantly enriched. In conclusion, our findings suggest that repeated daily exposure to low-dose DEPs can trigger an immune response via IL-1α and IL-17 signaling pathways during the early stages of PHNE maturation and further affect ciliogenesis by disrupting cilia assembly and motility during later stage of PHNE maturation.
HostingRepository	PRIDE
AnnounceDate	2026-05-23
AnnouncementXML	Submission_2026-05-23_14:41:08.353.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dohyun Han
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-09 03:05:34	ID requested
⏵ 1	2026-05-23 14:41:09	announced

10.1016/j.envres.2026.123849;

Han D, Choi J, Park MK, Kim N, Han DH, Inflammatory responses and cilia reorganization induced by daily exposure to diesel exhaust particles in primary human nasal epithelium. Environ Res, 294():123849(2026) [pubmed]

submitter keyword: Diesel exhaust particles, human nasal epithelium, TMT

Dohyun Han
contact affiliation	Proteomics Core Facility, Biomedicial Research Institute, Seoul National University Hospital
contact email	hdh03@snu.ac.kr
lab head
Dohyun Han
contact affiliation	Proteomics Core Facility, Biomedical Research Institute, Seoul National University Hospital
contact email	hdh03@snu.ac.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065940
     1. Label: PRIDE project
     2. Name: In depth proteome analyais of primary human nasal epithelium induced by diesel exhaust particles