PXD065124 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Comparative Proteomic Analysis of Paraquat and Alpha Synuclein Neurotoxicity Models in Drosophila |
| Description | In this project, we sought to perform proteomics in two Drosophila models: neuronally expressed human alpha synuclein model of parkinsonism and a paraquat neurotoxicity model. We exposed wild type Drosophila to 5 mM Paraquat in their food (potato starch flakes) for 7 days post-eclosion (i.e. in adult stage, not larvae). In a separate group, we expressed human alpha synuclein neuronally using the Q-system in the synaptobrevin promoter. Paraquat is a non-selective herbicide used for defoliation of green plant matter prior to harvesting and prolonged exposure has been epidemiologically associated with increased risk of developing Parkinson's Disease (PD). Alpha synuclein is a protein enriched in post-mortem brains of PD patients, primarily in protein aggregates known as Lewy bodies and Lewy neurites that are believed to be central to PD pathophysiology. PD is primarily associated with the progressive loss of dopaminergic neurons in the midbrain, however research has shown that prodromal symptoms (e.g. constipation, lowered gut motility, bladder disturbances) arise years prior to locomotor symptoms. Therefore, we separated the heads and bodies of our paraquat treated and alpha synuclein expressing flies and ran proteomics on each. These data were compared to determine if environmentally induced PD and genetically induced PD share common pathogenic mechanisms and if these changes are more pronounced in the brain or in the body. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-09-02 |
| AnnouncementXML | Submission_2026-09-02_10:12:22.125.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Pablo Reina-Gonzalez |
| SpeciesList | scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: NEWT:7227; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-06-17 11:31:12 | ID requested | |
| ⏵ 1 | 2026-09-02 10:12:22 | announced | |
Publication List
Keyword List
| submitter keyword: Heads, Bodies,Drosophila, Paraquat, Proteomics |
Contact List
| Souvarish Sarkar |
|---|
| contact affiliation | Envi-Gene Lab, Department of Environmental Medicine, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA |
| contact email | Souvarish_Sarkar@urmc.rochester.edu |
| lab head | |
| Pablo Reina-Gonzalez |
|---|
| contact affiliation | University of Rochester School of Medicine and Dentistry Department of Environmental Medicine |
| contact email | pablo_reinagonzalez@urmc.rochester.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD065124 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD065124
- Label: PRIDE project
- Name: Comparative Proteomic Analysis of Paraquat and Alpha Synuclein Neurotoxicity Models in Drosophila
