In this project, we sought to perform proteomics in two Drosophila models: neuronally expressed human alpha synuclein model of parkinsonism and a paraquat neurotoxicity model. We exposed wild type Drosophila to 5 mM Paraquat in their food (potato starch flakes) for 7 days post-eclosion (i.e. in adult stage, not larvae). In a separate group, we expressed human alpha synuclein neuronally using the Q-system in the synaptobrevin promoter. Paraquat is a non-selective herbicide used for defoliation of green plant matter prior to harvesting and prolonged exposure has been epidemiologically associated with increased risk of developing Parkinson's Disease (PD). Alpha synuclein is a protein enriched in post-mortem brains of PD patients, primarily in protein aggregates known as Lewy bodies and Lewy neurites that are believed to be central to PD pathophysiology. PD is primarily associated with the progressive loss of dopaminergic neurons in the midbrain, however research has shown that prodromal symptoms (e.g. constipation, lowered gut motility, bladder disturbances) arise years prior to locomotor symptoms. Therefore, we separated the heads and bodies of our paraquat treated and alpha synuclein expressing flies and ran proteomics on each. These data were compared to determine if environmentally induced PD and genetically induced PD share common pathogenic mechanisms and if these changes are more pronounced in the brain or in the body.