PXD065051-1

PXD065051 is an original dataset announced via ProteomeXchange.

Title	Host Proteins Interacting with Wild-Type and Signal Peptide-Cleaved GP64 of Bombyx mori Nucleopolyhedrovirus (BmNPV)
Description	Bombyx mori nucleopolyhedrovirus (BmNPV) relies on the envelope glycoprotein GP64 to mediate host cell entry. Intriguingly, both the wild-type GP64 retaining its signal peptide (SP) and the SP-cleaved variant (SP∆nGP64) can support viral invasion, yet their mechanisms may differ. To identify potential GP64 receptors and dissect host interaction profiles, we performed comparative co-immunoprecipitation using purified GP64 and SP∆nGP64, followed by data-independent acquisition (DIA)-based proteomic analysis. A total of 6,064 and 5,247 host proteins were identified, respectively. Enrichment analyses revealed shared involvement in membrane trafficking, lipid metabolism, and endocytosis—pathways essential for viral entry.
HostingRepository	PRIDE
AnnounceDate	2025-08-31
AnnouncementXML	Submission_2025-08-31_00:34:47.314.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kai Chen
SpeciesList	scientific name: Bombyx mori (Silk moth); NCBI TaxID: 7091;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-16 07:07:44	ID requested
⏵ 1	2025-08-31 00:34:47	announced

10.1016/J.MICPATH.2025.108004;

submitter keyword: proteomics, GP64, Envelope Protein-Host Interaction,BmNPV, signal peptide

Jinshan Huang
contact affiliation	Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu, People’s Republic of China Key Laboratory of Genetic Improvement of Sericulture in the Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of Agricultural Science, Zhenjiang, Jiangsu, People’s Republic of China
contact email	jshuang@just.edu.cn
lab head
Kai Chen
contact affiliation	Jiangsu University of Science and Technology
contact email	ckaiwork@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD065051

PRIDE project URI

• PRIDE
  1. PXD065051
     1. Label: PRIDE project
     2. Name: Host Proteins Interacting with Wild-Type and Signal Peptide-Cleaved GP64 of Bombyx mori Nucleopolyhedrovirus (BmNPV)