Bombyx mori nucleopolyhedrovirus (BmNPV) relies on the envelope glycoprotein GP64 to mediate host cell entry. Intriguingly, both the wild-type GP64 retaining its signal peptide (SP) and the SP-cleaved variant (SP∆nGP64) can support viral invasion, yet their mechanisms may differ. To identify potential GP64 receptors and dissect host interaction profiles, we performed comparative co-immunoprecipitation using purified GP64 and SP∆nGP64, followed by data-independent acquisition (DIA)-based proteomic analysis. A total of 6,064 and 5,247 host proteins were identified, respectively. Enrichment analyses revealed shared involvement in membrane trafficking, lipid metabolism, and endocytosis—pathways essential for viral entry.