PXD064361-1

PXD064361 is an original dataset announced via ProteomeXchange.

Title	Paleoproteomics characterization of fossil holobiont in acari-host-parasite interactions
Description	Paleoproteomics approaches the study of proteins by proteomics analysis in fossil samples. Recently, a protocol was developed and validated for paleoproteomics characterization of fossil arthropod parasitiformes in amber inclusions. In this study, the paleoproteomics approach was applied to arthropod fossil amber inclusions to characterize fossil holobiont with possible interactions between different organisms. The study targeted two arachnid fossil inclusions in Burmese amber (also known as Burmite or Kachin amber Cretaceous, ca. 99 mya). Although details of the dorsal and ventral idiosoma are equivocal, the results supported that fossils appear to belong to mites Prostigmata (Acari:Trombidiformes:Tetranychidae). Paleoproteomics analysis included peptide false-discovery rate (FDR), E-value of identified sequences in the UniProt databases with betweenness - PageRank centrality range (PR > 0.40) in phylogenetic analysis, and protein assigned score (PAS = E-value x PR). The analysis identified six Ubiquitin-like, Ig-like domain-containing and Peroxiredoxin-like proteins associated with mite species, thus supporting morphological findings. Ubiquitin-like proteins were also identified in ticks with similar sequence and structure to mite-derived protein. Other identified peptides were assigned to Ubiquitin-like proteins in birds and a parasitic nematode. The results suggested ancient holobiont composition with mite-bird-parasite interactions. The results are supported by ancient and modern interactions between these organisms. Despite current paleoproteomics limitations under investigation, these results advanced the characterization of fossil proteins relevant for the molecular evolution of Parasitiformes and the fossil holobiont in acari-host-parasite interactions.
HostingRepository	PRIDE
AnnounceDate	2026-03-23
AnnouncementXML	Submission_2026-03-23_03:31:59.480.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD064361
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Margarita Villar
SpeciesList	scientific name: fossil metagenome; NCBI TaxID: NEWT:444079;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-28 02:27:33	ID requested
⏵ 1	2026-03-23 03:31:59	announced

10.6019/PXD064361;

submitter keyword: amber

bird

arachnid

fossil

mite

paleoproteomics

parasitic nematode

Margarita Villar
contact affiliation	1 SaBio, Instituto de Investigación en Recursos Cinegéticos (IREC), Consejo Superior de Investigaciones Científicas (CSIC), Universidad de Castilla-La Mancha (UCLM)-Junta de Comunidades de Castilla-La Mancha (JCCM), Ronda de Toledo 12, 13005 Ciudad Real, Spain
contact email	margaritam.villar@uclm.es
lab head
Margarita Villar
contact affiliation	University of Castilla- La Mancha
contact email	margaritam.villar@uclm.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD064361

PRIDE project URI

• PRIDE
  1. PXD064361
     1. Label: PRIDE project
     2. Name: Paleoproteomics characterization of fossil holobiont in acari-host-parasite interactions