Paleoproteomics approaches the study of proteins by proteomics analysis in fossil samples. Recently, a protocol was developed and validated for paleoproteomics characterization of fossil arthropod parasitiformes in amber inclusions. In this study, the paleoproteomics approach was applied to arthropod fossil amber inclusions to characterize fossil holobiont with possible interactions between different organisms. The study targeted two arachnid fossil inclusions in Burmese amber (also known as Burmite or Kachin amber Cretaceous, ca. 99 mya). Although details of the dorsal and ventral idiosoma are equivocal, the results supported that fossils appear to belong to mites Prostigmata (Acari:Trombidiformes:Tetranychidae). Paleoproteomics analysis included peptide false-discovery rate (FDR), E-value of identified sequences in the UniProt databases with betweenness - PageRank centrality range (PR > 0.40) in phylogenetic analysis, and protein assigned score (PAS = E-value x PR). The analysis identified six Ubiquitin-like, Ig-like domain-containing and Peroxiredoxin-like proteins associated with mite species, thus supporting morphological findings. Ubiquitin-like proteins were also identified in ticks with similar sequence and structure to mite-derived protein. Other identified peptides were assigned to Ubiquitin-like proteins in birds and a parasitic nematode. The results suggested ancient holobiont composition with mite-bird-parasite interactions. The results are supported by ancient and modern interactions between these organisms. Despite current paleoproteomics limitations under investigation, these results advanced the characterization of fossil proteins relevant for the molecular evolution of Parasitiformes and the fossil holobiont in acari-host-parasite interactions.