PXD062958-1

PXD062958 is an original dataset announced via ProteomeXchange.

Title	Identification of Stroke Biomarkers Using Proteomic Profiling of Extracellular Vesicles Derived from Human Blood: A Preliminary Study
Description	Stroke is a devastating brain disease that causes extensive neurological impairment and high mortality. Rapid diagnosis and intervention of stroke are necessary to minimize neurological damage and improve recovery. Extracellular vesicles (EVs) have been identified as potential biomarkers for stroke, suggesting promising avenues for rapid diagnosis and prognostic assessments. This preliminary study aimed to evaluate the potential of EVs as biomarkers in the distinct pathophysiological mechanisms of hemorrhagic stroke (HS) and ischemic strokes (IS). We have identified proteins differentially expressed in EVs derived from the blood of HS and IS patients. EVs were isolated using an isolation kit, followed by proteomic analysis by LC-MS/MS to compare protein expression patterns. As a result of the proteomics analysis of blood-derived EVs, 15 proteins were upregulated and 4 downregulated in EVs from HS patients. Similarly, 14 proteins were upregulated and 5 downregulated in EVs from IS patients. Notably, 4 proteins were commonly upregulated and 1 protein was commonly downregulated across both conditions, with the remaining proteins uniquely altered in each type of stroke. To validate the proteomic findings, we confirmed the increased levels of CRP and PF4 in HS patients using ELISA, verifying their elevation in patient blood samples. These findings indicate that the proteins identified in blood-derived EVs hold the potential as biomarkers for HS, highlighting their utility for clinical application.
HostingRepository	PRIDE
AnnounceDate	2025-06-10
AnnouncementXML	Submission_2025-06-10_04:00:34.754.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kim Tae Yeon
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	carbamoylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-14 21:55:37	ID requested
⏵ 1	2025-06-10 04:00:35	announced

10.3389/FNEUR.2025.1587389;

submitter keyword: ischemic stroke, extracellular vesicles,hemorrhagic stroke, proteomics., biomarker

Tae Yeon Kim
contact affiliation	Department of Physical Education, Seoul National University, Seoul, Republic of Korea, 08826
contact email	tyeonkim@snu.ac.kr
lab head
Kim Tae Yeon
contact affiliation	Seoul National University
contact email	tyeonkim@snu.ac.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062958
     1. Label: PRIDE project
     2. Name: Identification of Stroke Biomarkers Using Proteomic Profiling of Extracellular Vesicles Derived from Human Blood: A Preliminary Study