Stroke is a devastating brain disease that causes extensive neurological impairment and high mortality. Rapid diagnosis and intervention of stroke are necessary to minimize neurological damage and improve recovery. Extracellular vesicles (EVs) have been identified as potential biomarkers for stroke, suggesting promising avenues for rapid diagnosis and prognostic assessments. This preliminary study aimed to evaluate the potential of EVs as biomarkers in the distinct pathophysiological mechanisms of hemorrhagic stroke (HS) and ischemic strokes (IS). We have identified proteins differentially expressed in EVs derived from the blood of HS and IS patients. EVs were isolated using an isolation kit, followed by proteomic analysis by LC-MS/MS to compare protein expression patterns. As a result of the proteomics analysis of blood-derived EVs, 15 proteins were upregulated and 4 downregulated in EVs from HS patients. Similarly, 14 proteins were upregulated and 5 downregulated in EVs from IS patients. Notably, 4 proteins were commonly upregulated and 1 protein was commonly downregulated across both conditions, with the remaining proteins uniquely altered in each type of stroke. To validate the proteomic findings, we confirmed the increased levels of CRP and PF4 in HS patients using ELISA, verifying their elevation in patient blood samples. These findings indicate that the proteins identified in blood-derived EVs hold the potential as biomarkers for HS, highlighting their utility for clinical application.