PXD061718-1

PXD061718 is an original dataset announced via ProteomeXchange.

Title	Protein interactome of HNRNPU in differentiating neural cells
Description	HNRNPU is an RNA-binding protein with diverse roles in regulating gene expression. Pathogenic genetic variants of HNRNPU cause a severe neurodevelopmental disorder (NDD), but the underlying molecular mechanisms are unclear. Here, we investigated the protein-protein interaction (PPI) network of HNRNPU in neuroepithelial stem cells (NES) and differentiating neural cells derived from human induced pluripotent stem cells. Using high-throughput approaches, we found HNRNPU interacting with the mammalian SWI/SNF complex and highlight its putative role in multiple stages of mRNA regulation. Notably, both PPI partners and interacting mRNAs implicated HNRNPU in translation, a role that had not been recognized before.
HostingRepository	PRIDE
AnnounceDate	2026-02-23
AnnouncementXML	Submission_2026-02-22_17:50:35.950.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marika Oksanen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-11 11:57:29	ID requested
⏵ 1	2026-02-22 17:50:37	announced

10.1093/nar/gkag107;

Oksanen M, Mastropasqua F, Mazan-Mamczarz K, Martindale JL, Ye X, Arora A, Banskota N, Gorospe M, Tammimies K, Molecular interactome of HNRNPU reveals regulatory networks in neuronal differentiation and DNA methylation. Nucleic Acids Res, 54(4):(2026) [pubmed]

submitter keyword: Human, differentiation, neural cell, LC-ESI-MS/MS

Kristiina Tammimies
contact affiliation	Center of Neurodevelopmental Disorders (KIND), Centre for Psychiatry Research, Department of Women’s and Children’s Health, Karolinska Institute, Region Stockholm, Stockholm, Sweden Astrid Lindgren Children’s Hospital, Karolinska University Hospital, Region Stockholm, Stockholm, Sweden
contact email	kristiina.tammimies@ki.se
lab head
Marika Oksanen
contact affiliation	Karolinska Institutet
contact email	marika.oksanen@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD061718

PRIDE project URI

• PRIDE
  1. PXD061718
     1. Label: PRIDE project
     2. Name: Protein interactome of HNRNPU in differentiating neural cells