PXD061521-2

PXD061521 is an original dataset announced via ProteomeXchange.

Title	RNF25-Mediated NF-κB Activation Drives Anti-Apoptosis and Therapy Resistance in Cancer
Description	Dysregulation of the NF-κB pathway is frequently observed in cancers, where it contributes to therapy resistance by inhibiting apoptosis. Mass spectrometry (MS)-based proteomics were employed to identify factors associated with the anti-apoptotic process. We identify RNF25, an E3 ubiquitin ligase, as a novel anti-apoptotic factor in renal cancer cells. RNF25 interacts with TRIP4 and promotes its non-degradative ubiquitination at lysine 135, preventing TRIP4 from binding to p65. This action releases p65, activating the NF-κB pathway and leading to the increased expression of anti-apoptotic genes like cIAP2 and Bcl-2.
HostingRepository	iProX
AnnounceDate	2025-03-05
AnnouncementXML	Submission_2025-09-11_20:08:35.457.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lei Li
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	ubiquitination signature dipeptidyl lysine
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-05 20:11:50	ID requested
1	2025-08-31 20:43:29	announced
⏵ 2	2025-09-11 20:08:36	announced	2025-09-12: Add info

Li L, Wang Z, Ma B, Ye Q, Lei Y, Lu M, Ye L, Kang J, Huang W, Xu S, Wang K, Chen Y, Liu J, Gao Y, Wang C, Ma J, Li L, B Activation and Apoptosis Resistance in Renal Cell Carcinoma. Int J Biol Sci, 21(10):4410-4427(2025) [pubmed]

submitter keyword: anti-apoptosis, NF-κB activation, RNF25, TRIP4, therapy resistance

Lei Li
contact affiliation	Department of Urology, The First Affiliated Hospital of Xi'an Jiaotong University
contact email	lilydr@163.com
lab head
Lei Li
contact affiliation	Department of Urology, The First Affiliated Hospital of Xi'an Jiaotong University
contact email	1119814379@qq.com
dataset submitter

iProX dataset URI