PXD061521-1

PXD061521 is an original dataset announced via ProteomeXchange.

Title	RNF25-Mediated NF-κB Activation Drives Anti-Apoptosis and Therapy Resistance in Cancer
Description	Dysregulation of the NF-κB pathway is frequently observed in cancers, where it contributes to therapy resistance by inhibiting apoptosis. Mass spectrometry (MS)-based proteomics were employed to identify factors associated with the anti-apoptotic process. We identify RNF25, an E3 ubiquitin ligase, as a novel anti-apoptotic factor in renal cancer cells. RNF25 interacts with TRIP4 and promotes its non-degradative ubiquitination at lysine 135, preventing TRIP4 from binding to p65. This action releases p65, activating the NF-κB pathway and leading to the increased expression of anti-apoptotic genes like cIAP2 and Bcl-2.
HostingRepository	iProX
AnnounceDate	2025-03-05
AnnouncementXML	Submission_2025-08-31_20:43:29.102.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lei Li
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	ubiquitination signature dipeptidyl lysine
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-05 20:11:50	ID requested
⏵ 1	2025-08-31 20:43:29	announced
2	2025-09-11 20:08:36	announced	2025-09-12: Add info

Dataset with its publication pending

submitter keyword: anti-apoptosis, NF-κB activation, RNF25, TRIP4, therapy resistance

Lei Li
contact affiliation	Department of Urology, The First Affiliated Hospital of Xi'an Jiaotong University
contact email	lilydr@163.com
lab head
Lei Li
contact affiliation	Department of Urology, The First Affiliated Hospital of Xi'an Jiaotong University
contact email	1119814379@qq.com
dataset submitter

iProX dataset URI