PXD061353-1

PXD061353 is an original dataset announced via ProteomeXchange.

Title	Exoproteome of clinical isolates of Pseudomonas aeruginosa with limited cheating ability
Description	Pseudomonas aeruginosa is an opportunistic bacterial pathogen that possesses a plethora of virulence factors, including the public goods siderophores and exoproteases. Mutants unable to produce either exoprotease or siderophores are selected in competition with producers; hence, these two virulence factors are prone to exploitation by social cheaters. However, so far most of the studies of exoprotease exploitation by social cheaters had been done in the reference strains PAO1 and PA14 and the information available of these behaviors in clinical or environmental isolates is limited to a few studies. In this work, we studied the exoproteases secreted by two clinical isolates from cystic fibrosis patients belonging to the epidemic clone ST274-CC274. We found that the exoprotease exploitation in these strains is mild and not a result of the selection of lasR mutants during continuous growth in casein as sole carbon source, in contrast with the paradigm in the reference strains.
HostingRepository	PRIDE
AnnounceDate	2025-11-10
AnnouncementXML	Submission_2025-11-09_16:12:40.782.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD061353
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hector Quezada
SpeciesList	scientific name: Pseudomonas aeruginosa PAO1H2O; NCBI TaxID: NEWT:1147787; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationList	iodoacetamide derivatized residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-01 13:43:56	ID requested
⏵ 1	2025-11-09 16:12:41	announced

Guadarrama-Orozco KD, Esquivel-Hern, á, ndez DA, Islas-Tolentino M Á, Husain FM, Quezada H, Garc, í, a-Reyes S, Franco B, Marroquin-Mendiola DL, Lucero-Gil MG, Olvera-Falfan LP, Estrada-Velasco Á Y, Fabi, á, n Del Olmo MJ, Cocotl-Ya, ñ, ez M, Tomas M, Pe, ñ, a-Oca, ñ, a BA, Maeda T, Khan A, Arshad M, Cant, ó, n R, Oliver A, Kidd TJ, Valdez A, Cadet F, Toya S, Fontaine N, Ceap, ă CD, Kirigo J, Wood TK, Garc, í, a-Contreras R, Challenging the paradigm: non-canonical exoprotease cheating in clinical Pseudomonas aeruginosa isolates. FEMS Microbiol Ecol, 101(11):(2025) [pubmed]

10.6019/PXD061353;

10.1093/femsec/fiaf106;

submitter keyword: population collapse,Exoproteases , bacteria, quorum-sensing

Rodolfo Garcia Contreras
contact affiliation	Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de México 04510, Mexico; rgarc@bq.unam.mx
contact email	hquezada@himfg.edu.mx
lab head
Hector Quezada
contact affiliation	Hospital Infantil de Mexico Federico Gomez
contact email	hquezada@himfg.edu.mx
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD061353

PRIDE project URI

• PRIDE
  1. PXD061353
     1. Label: PRIDE project
     2. Name: Exoproteome of clinical isolates of Pseudomonas aeruginosa with limited cheating ability