PXD059617-1

PXD059617 is an original dataset announced via ProteomeXchange.

Title	Lactate uptake and oxidation via mitochondrial CD147/MCT1/LDHB complex contributes to stemness potentials in metastatic breast cancer
Description	Metastatic cancer cells, originating from cancer stem cells with metastatic capacity, utilize nutrient flexibility to overcome the hurdles of metastatic cascade. However, the nutrient supply for maintaining the stemness potentials of metastatic cancer cells remains unknown. Here, we revealed that metastatic breast cancer cells maintain stemness and initiate metastasis upon detachment via uptaking and oxidating lactate. In detached metastasizing breast cancer cells, lactate was incorporated into tricarboxylic acid cycle and boosted oxidative phosphorylation, and then promoted the stemness potentials via α-KG-DNMT3B-mediated SOX2 hypomethylation. Moreover, lactate was uptake and oxidated in mitochondria by CD147/MCT1/LDHB complex, whose existence correlates to the stemness potentials and tumor metastasis in breast cancer patients. An intracellularly expressed single chain variable fragment targeting mitochondrial CD147 (mito-CD147 scFv) effectively disrupted mitochondrial CD147/MCT1/LDHB complex, inhibited lactate-induced stemness potentials, depleted circulating breast cancer cells and reduced metastatic burden, suggesting a promising clinical application in reducing lactate-fueled metastasis.
HostingRepository	iProX
AnnounceDate	2025-01-09
AnnouncementXML	Submission_2025-01-09_23:33:24.724.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiajia Zhang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-09 23:33:13	ID requested
⏵ 1	2025-01-09 23:33:25	announced

Dataset with its publication pending

submitter keyword: metastatic cancer cells, stemness potentials, detachment, lactate, mitochondria, CD147/MCT1/LDHB complex, mito-CD147 scFv

Ling Li
contact affiliation	Fourth Military Medical University
contact email	liling25@fmmu.edu.cn
lab head
Jiajia Zhang
contact affiliation	Fourth Military Medical University
contact email	1240568116@qq.com
dataset submitter

iProX dataset URI