PXD057717-1

PXD057717 is an original dataset announced via ProteomeXchange.

Title	mice-development-stages-platelets-proteomics
Description	Platelet proteome samples were prepared as previously described[Wang, H., Liu, C., et al., Immunity, 2023]. Briefly, the purified platelet precipitate was resuspended by protein lysis and protein cleavage was carried out with 8M Urea. Then the enzymatic hydrolysis and peptide recovery were carried out with trypsin. Fractionation was performed by high pH reverse HPLC and then separation was performed by EASY-nLC 1200 ultra-high performance liquid phase system. Data acquisition was performed in data-dependent mode using Orbitrap Exploris 480 mass spectrometry. The peptide parent ion and its secondary fragments were detected and analyzed with Orbitrap Exploris 480 at high resolution.The raw data of mouse platelet proteome were searched and processed using MaxQuant software (v1.6.15.0) in conjunction with the UniProt protein database to generate a protein expression matrix.
HostingRepository	iProX
AnnounceDate	2024-11-11
AnnouncementXML	Submission_2025-06-24_19:17:06.332.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Le Li
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-10 20:01:30	ID requested
⏵ 1	2025-06-24 19:17:06	announced

Dataset with its publication pending

submitter keyword: mice,development,platelets

Le Li
contact affiliation	Chinese Academy of Medical Sciences
contact email	lile_bioinfor@163.com
lab head
Le Li
contact affiliation	Chinese Academy of Medical Sciences
contact email	lile_bioinfor@163.com
dataset submitter

iProX dataset URI