Platelet proteome samples were prepared as previously described[Wang, H., Liu, C., et al., Immunity, 2023]. Briefly, the purified platelet precipitate was resuspended by protein lysis and protein cleavage was carried out with 8M Urea. Then the enzymatic hydrolysis and peptide recovery were carried out with trypsin. Fractionation was performed by high pH reverse HPLC and then separation was performed by EASY-nLC 1200 ultra-high performance liquid phase system. Data acquisition was performed in data-dependent mode using Orbitrap Exploris 480 mass spectrometry. The peptide parent ion and its secondary fragments were detected and analyzed with Orbitrap Exploris 480 at high resolution.The raw data of mouse platelet proteome were searched and processed using MaxQuant software (v1.6.15.0) in conjunction with the UniProt protein database to generate a protein expression matrix.